1. Female patients over 35 years old;

2. Patients with recurrent spontaneous abortion  or unexplained abortion;

3. Coupless with chromosomal abnormalities;

4. Couples with repeated failure of IVF;

5. Couples with abnormal chromosome number and/or structure, such as balanced translocation, partial cretinism, etc;

6. Couples with single gene disease or carrier on one or both sides;